Signalling

Part:BBa_K091135

Designed by: Erin Feeney   Group: iGEM08_Davidson-Missouri_Western   (2008-07-14)


Las Sender Testing Device

K091000 constitutively produces LasI, the autoinducer synthase for PAI-1, to test Las quorum sensing signaling. This part consists of the promoter J2100, an RBS, and the LasI gene (without an LVA tag).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None